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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
02/07/2021 |
Data da última atualização: |
02/07/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
PAZ VENTERO, M.; CUADRAT, R. R. C.; VIDAL, I.; ANDRADE, B. G. N.; MOLINA-PARDINES, C.; HARI-MORENO, J. M.; COUTINHO, F. H.; MERINO, E.; REGITANO, L. C. de A.; SILVEIRA, C. B.; AFLI, H.; LÓPEZ-PÉREZ, M.; RODRÍGUEZ, J. C. |
Afiliação: |
MARIA PAZ VENTERO, ISABIAL; RAFAEL R. C. CUADRAT, German Institute of Human Nutrition Potsdam-Rehbrücke; INMACULADA VIDAL, ISABIAL; BRUNO G. N. ANDRADE, Munster Technological University; CARMEN MOLINA-PARDINES, ISABIAL; JOSE M. HARO-MORENO, Universidad Miguel Hernández; FELIPE H. COUTINHO, Universidad Miguel Hernández; ESPERANZA MERINO, ISABIAL; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; CYNTHIA B. SILVEIRA, University of Miami; HAITHEM AFLI, Munster Technological University; MARIO LÓPEZ-PÉREZ, ISABIAL; JUAN CARLOS RODRÍGUEZ, ISABIAL. |
Título: |
Nasopharyngeal microbial communities of patients infected with SARS-CoV-2 that developed COVID-19. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Frontiers in Microbiology, v.12, 637430, mar. 2021 |
Páginas: |
10 p. |
DOI: |
10.3389/fmicb.2021.637430 |
Idioma: |
Inglês |
Conteúdo: |
Background: SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient?s nasopharynx microbiota. In this study we investigated the nasopharynx microbial community of patients that developed different severity levels of COVID-19. We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained from SARS-CoV-2 positive (56) and negative (18) patients in the province of Alicante (Spain) in their first visit to the hospital. Positive SARS-CoV-2 patients were observed and later categorized in mild (symptomatic without hospitalization), moderate (hospitalization), and severe (admission to ICU). We compared the microbiota diversity and OTU composition among severity groups and built bacterial co-abundance networks for each group. Results: Statistical analysis indicated differences in the nasopharyngeal microbiome of COVID19 patients. 62 OTUs were found exclusively in SARS-CoV-2 positive patients, mostly classified as members of the phylum Bacteroidota (18) and Firmicutes (25). OTUs classified as Prevotella were found to be significantly more abundant in patients that developed more severe COVID-19. Furthermore, co-abundance analysis indicated a loss of network complexity among samples from patients that later developed more severe symptoms. Conclusion: Our study shows that the nasopharyngeal microbiome of COVID-19 patients showed differences in the composition of specific OTUs and complexity of co-abundance networks. Taxa with differential abundances among groups could serve as biomarkers for COVID-19 severity. Nevertheless, further studies with larger sample sizes should be conducted to validate these results. MenosBackground: SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient?s nasopharynx microbiota. In this study we investigated the nasopharynx microbial community of patients that developed different severity levels of COVID-19. We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained from SARS-CoV-2 positive (56) and negative (18) patients in the province of Alicante (Spain) in their first visit to the hospital. Positive SARS-CoV-2 patients were observed and later categorized in mild (symptomatic without hospitalization), moderate (hospitalization), and severe (admission to ICU). We compared the microbiota diversity and OTU composition among severity groups and built bacterial co-abundance networks for each group. Results: Statistical analysis indicated differences in the nasopharyngeal microbiome of COVID19 patients. 62 OTUs were found exclusively in SARS-CoV-2 positive patients, mostly classified as members of the phylum Bacteroidota (18) and Firmicutes (25). OTUs classified as Prevotella were found to be significantly more abundant in patients that developed more severe COVID-19. Furthermore, co-abundance analysis indicated a loss of network complexity among samples from patients that later developed more severe symptoms. Conclusion: Our study shows that the nasopharyngeal microbiome of COVID-19 patients showed d... Mostrar Tudo |
Palavras-Chave: |
Coronavirus; COVID 19; NGS next generation sequencing; SARS CoV 2. |
Thesaurus Nal: |
Microbiome; Prevotella. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/224271/1/NasopharyngealMicrobialCommunities.pdf
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Marc: |
LEADER 02818naa a2200361 a 4500 001 2132766 005 2021-07-02 008 2021 bl uuuu u00u1 u #d 024 7 $a10.3389/fmicb.2021.637430$2DOI 100 1 $aPAZ VENTERO, M. 245 $aNasopharyngeal microbial communities of patients infected with SARS-CoV-2 that developed COVID-19.$h[electronic resource] 260 $c2021 300 $a10 p. 520 $aBackground: SARS-CoV-2 is an RNA virus causing COVID-19. The clinical characteristics and epidemiology of COVID-19 have been extensively investigated, however, only one study so far focused on the patient?s nasopharynx microbiota. In this study we investigated the nasopharynx microbial community of patients that developed different severity levels of COVID-19. We performed 16S ribosomal DNA sequencing from nasopharyngeal swab samples obtained from SARS-CoV-2 positive (56) and negative (18) patients in the province of Alicante (Spain) in their first visit to the hospital. Positive SARS-CoV-2 patients were observed and later categorized in mild (symptomatic without hospitalization), moderate (hospitalization), and severe (admission to ICU). We compared the microbiota diversity and OTU composition among severity groups and built bacterial co-abundance networks for each group. Results: Statistical analysis indicated differences in the nasopharyngeal microbiome of COVID19 patients. 62 OTUs were found exclusively in SARS-CoV-2 positive patients, mostly classified as members of the phylum Bacteroidota (18) and Firmicutes (25). OTUs classified as Prevotella were found to be significantly more abundant in patients that developed more severe COVID-19. Furthermore, co-abundance analysis indicated a loss of network complexity among samples from patients that later developed more severe symptoms. Conclusion: Our study shows that the nasopharyngeal microbiome of COVID-19 patients showed differences in the composition of specific OTUs and complexity of co-abundance networks. Taxa with differential abundances among groups could serve as biomarkers for COVID-19 severity. Nevertheless, further studies with larger sample sizes should be conducted to validate these results. 650 $aMicrobiome 650 $aPrevotella 653 $aCoronavirus 653 $aCOVID 19 653 $aNGS next generation sequencing 653 $aSARS CoV 2 700 1 $aCUADRAT, R. R. C. 700 1 $aVIDAL, I. 700 1 $aANDRADE, B. G. N. 700 1 $aMOLINA-PARDINES, C. 700 1 $aHARI-MORENO, J. M. 700 1 $aCOUTINHO, F. H. 700 1 $aMERINO, E. 700 1 $aREGITANO, L. C. de A. 700 1 $aSILVEIRA, C. B. 700 1 $aAFLI, H. 700 1 $aLÓPEZ-PÉREZ, M. 700 1 $aRODRÍGUEZ, J. C. 773 $tFrontiers in Microbiology$gv.12, 637430, mar. 2021
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
08/08/2001 |
Data da última atualização: |
17/05/2019 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
ANJOS, J. B. dos. |
Afiliação: |
JOSE BARBOSA DOS ANJOS, CPATSA. |
Título: |
Mecanizacao agricola, manejo e conservacao de solo. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: LEAO, P. C. de S.; SOARES, J. M. (Ed.). A viticultura no Semi-Árido brasileiro. Petrolina: Embrapa Semi-Arido, 2000. |
Páginas: |
cap. 10, p. 259-272. |
Descrição Física: |
il. |
Idioma: |
Português |
Conteúdo: |
ESCOLHA DA ÁREA; PREPARO INICIAL DO SOLO; LEVANTAMENTO DA ÁREA; PREPARO DO SOLO PARA IMPLANTAÇÃO DO POMAR; MÉTODOS DE IRRIGAÇÃO; TRATOS CULTURAIS; TRÁFEGO DE MÁQUINAS; MANEJO DE SOLO. |
Palavras-Chave: |
Agricultural mechanization; Conservation; Management; Soils. |
Thesagro: |
Conservação; Manejo; Mecanização Agrícola; Solo; Uva. |
Thesaurus NAL: |
grapes. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/197422/1/A-Viticultura-no-Semi-Arido-pag-259-273.pdf
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Marc: |
LEADER 00911naa a2200253 a 4500 001 1134244 005 2019-05-17 008 2000 bl uuuu u00u1 u #d 100 1 $aANJOS, J. B. dos 245 $aMecanizacao agricola, manejo e conservacao de solo. 260 $c2000 300 $acap. 10, p. 259-272.$cil. 520 $aESCOLHA DA ÁREA; PREPARO INICIAL DO SOLO; LEVANTAMENTO DA ÁREA; PREPARO DO SOLO PARA IMPLANTAÇÃO DO POMAR; MÉTODOS DE IRRIGAÇÃO; TRATOS CULTURAIS; TRÁFEGO DE MÁQUINAS; MANEJO DE SOLO. 650 $agrapes 650 $aConservação 650 $aManejo 650 $aMecanização Agrícola 650 $aSolo 650 $aUva 653 $aAgricultural mechanization 653 $aConservation 653 $aManagement 653 $aSoils 773 $tIn: LEAO, P. C. de S.; SOARES, J. M. (Ed.). A viticultura no Semi-Árido brasileiro. Petrolina: Embrapa Semi-Arido, 2000.
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